Streptozotocin is a glucosaminenitrosourea compound. Diabetic rats were treated daily for 12 weeks with lc 50 or 200 mgkgd intravenously. Characterisation of streptozotocin induced diabetes mellitus. Role of insulin, oxidative stress and nitric oxide nagy m a1, bastawy m a1, 2abdelhamid n m, 1chemistry department, faculty of science, benisuef university, egypt 2biochemistry department, faculty of pharmacy, miniauniversity, egypt. Effect of nigella sativa fixed and essential oils on. Superoxide dismutase was administered intravenously to rats 50min prior to intravenous. Stz is a nitrosurea compound derived from streptomyces achromogenes, which has also been used as an antibiotic and a cancer treatment. The livers were removed on either the 10th or the 30th day of the disease, weighed, and used to evaluate the activity, expression, and localization of lysosomal enzymes. Whether the increased synthesis and accumulation of vascular collagen is a cause or an effect of the elevated blood pressure in animal models of hypertension is still unresolved. Alloxan and streptozotocin are toxic glucose analogues that.
Stzinduced insulin deficiency and hyperglycemia in mice and rats. According to one published study, enhanced oxidative stress and damaged antioxidant defense systems were observed in patients with diabetes, seem to result in the ini tiation and development of various diabetes induced complications 2. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Streptozotocin stz is an alkylating agent that affects pancreatic islets, inducing diabetes in mice. The animals then receive injections of streptozotocin to induce insulindependent diabetes mellitus. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. After treatment, in the pancreas no insulitis was observed. Several other studies found that stzinduced diabetes can be treated by plant extracts which control the blood sugar level as well as improving the lipid profile. Unlock diy streptozotocin induced diabetes type 2 showing you easy to your type 2 diabetes recipes instead of replacing it. Evaluation of antidiabetic activity of ipomoea aquatica. Jun 17, 2014 nigella sativa fixed nsfo and essential nseo oils have been used to treat diabetes mellitus and its complications. Group 4 was injected with streptozotocin and later treated in the same way as in group 3 streptozotocin manganese group.
Streptozotocin high fat diet induced type 2 diabetes. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Black ginseng extract counteracts streptozotocininduced. Step by step guide to easily your type 2 diabetes recipes. The longterm effects of streptozotocin and possible recovery from streptozotocin induced diabetes are explored much less. These findings suggest that multiple injections of streptozotocin induce, in susceptible hosts, the triad of direct beta cell cytotoxicity, virus induction within beta cells, and cellmediated autoimmune reaction.
Previous studies in this laboratory have indicated that chronic streptozotocin stz induced diabetes 8 weeks. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Protection against streptozotocin induced diabetes by superoxide dismutase m. Diabetes was established by intraperitoneal stz injection 65 mgkg in sprague dawley rats. Streptozotocin is an antimicrobial agent and has also been used as a chemotherapeutic alkylating agent 67. Effect of streptozotocin on glucose levels in albino wister rats. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. Full text streptozotocininduced type 1 diabetes in rodents. Furthermore, streptozotocin induced diabetes caused a progressive loss of weight from 4 to 8 weeks and a. The streptozotocin induced diabetes model has been extensively used, making it particularly useful for building upon and comparing results of other studies. Streptozotocin use in mice and rats version 1 date of publication. Protective role of opn in the islets osteopontin opn is a secrete osteopontin opn is a secreted acidic phosphoprotein that binds to a cell surface integrinbinding motif and is involved in many inflammatory and immunemodulating disorders. Hypertension is associated with elevated rates of collagen synthesis and deposition in the arterial wall. Type 1 diabetes mellitus was induced in rats by the intraperitoneal injection of freshly prepared streptozotocin stz at a dose of 45 mgkg dissolved in 0.
Stz treatment successfully induced hyperglycemia in rats fig. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. Nov 28, 2012 data are preliminary on the hypoglycemic effect of carica papaya leaves in streptozotocin induced diabetic rats. Piper sarmentosum water extract attenuates diabetic. A 5060% decrease in the specific activities of cysteine proteases, especially cathepsin b, was observed in streptozotocin induced diabetes mellitus. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. This study has determined whether chronic diabetes induced by streptozotocin in rats mimics the complications associated with human diabetes. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp.
The user has requested enhancement of the downloaded file. High dose stz induces necrosis and multiple lowdose causes apoptosis in. Induction of diabetes mellitus in rats using intraperitoneal. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight. Changes in oxidative stress and antioxidant enzyme activities. Streptozotocininduced diabetes mellitus affects lysosomal. The male rats with average weight 200220 g were divided into four groups n6, control, diabetic induced with a single dose of streptozotocin 50 mgkg body. The chronic complications of diabetes in humans include cardiomyopathy, neuropathic pain, cataract development and retinopathy. Dec 18, 2007 figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected. Detailed in this unit are protocols for producing stz. Neural degeneration in the retina of the streptozotocin induced type 1 diabetes model. Ebscohost serves thousands of libraries with premium essays, articles and other content including induction of diabetes mellitus in rats using intraperitoneal streptozotocin. Chao qian, chenyuan zhu, weiqiang yu, xinquan jiang, fuqiang zhang.
Type 1 diabetes induced by stz is a dose related process. Unlike type 1 diabetes, type 2 diabetes t2d is associated with resistance to insulin action. Original article renoprotective effects of lcarnitine in. How to induce permanent diabetes by streptozotocin in wistar rats.
Treatment with streptozotocin increases blood glucose levels in mice by itself. Metabolic and vascular alterations induced by streptozotocin induced diabetes. Three days after the stz injection, the blood was withdrawn from the tail vein, and the glucose level. Studies of streptozotocininduced insulitis and diabetes. Original article crocin enhances antioxidative and. Burr department of pediatrics, vanderbilt university medical school, nashville, tennessee, usa summary. Diabetes induction in mice iacuc standard procedure effective. For severe diabetes induction, stz is administered at 4050 mgkg body weight. Stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. However, there is a lack of data on the efficacy of black ginseng in preventing diabetes mellitus.
Pdf isolated soy proteinbased diet ameliorates glycemia. Opposite effects of angiotensins receptors type 2 and type 4. Protection against streptozotocininduced diabetes by. Diabetes mellitus dm is a chronic disease characterized by. Streptozotocin stz induced diabetes mellitus dm offers a very cost effective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. It is interesting that fermented red ginseng, with a different ginsenoside profile, exhibited a strong antidiabetic effects in streptozotocin stz induced diabetic rats and type 2 diabetes patients. The rat is the most commonly used model of human disease. Highfat dietlowdose streptozotocininduced type 2 diabetes. The mechanisms of alloxan and streptozotocininduced diabetes. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Isolated soy proteinbased diet ameliorates glycemia and antioxidants enzyme activities in streptozotocininduced diabetes.
Beginning the day after the alloxan or stz injection, blood sugar levels of each mouse are checked daily or every other day for 57 days by following the glucose monitoring of blood procedure. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Multiple low dose streptozotocininduced diabetes in the rat. Diabetes is associated with declining sexual function in women. Hypoglycemic effect of carica papaya leaves in streptozotocin.
Diabetes was induced with streptozotocin, the standard and most common method for experimental diabetes 35, 36,41. Multiple low dose streptozotocin induced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Fvbnj 001800, balbcj 000651, and aj 000646 males are resistant. Streptozotocin induced diabetes in animal models ijppr. The higher the dose the greater the number of beta cells killed. Treatment with streptozotocin increases blood glucose levels in mice by itself before the septic challenge figure 1a. In this guide, well show you technique how do i streptozotocin induced diabetes type 2. Streptozotocinhigh fat dietinduced type 2 diabetes bienta. Pdf induction of diabetes by streptozotocin in rats. Streptozotocininduced diabetes in the rat is associated with.
However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Streptozotocin diabetes is caused by the specific necrosis of the pancreatic. Present study was undertaken to explore and validate these folkloric uses. Dec, 2009 streptozotocin is widely used in medical research for treating certain cancers of the islets of langerhans and to produce an animal model for type 1 diabetes. Many investigators conclude that stz produces type i diabetes mellitus 35. How to induce permanent diabetes by streptozotocin in. Sprague dawley rats having streptozotocin stz induced diabetes mellitus were used to assess the role of nsfo and nseo in the management of diabetes complications. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. Mulberry leaf alleviates streptozotocininduced diabetic rats by. How to induce permanent diabetes by streptozotocin in wistar.
Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Streptozotocininduced diabetic ratsmice stz model advantages. The pathological change of diabetes mellitus is complicated. However, there is a lack of data on the efficacy of black gin. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. Cessation of these preventive treatments was followed by the appearance of insulitis and diabetes. To develop the corresponding animal pathology model, high fat diet hfd is often used. Group 5 was administered with managanese for 2 wk, given streptozotocin and followed by 1 wk saline administration manganese streptozotocin saline group.
Previous reported literature indicates that the type of diabetes and characteristics differ with the employed dose of stz and animal and species used 47. Highfat dietlowdose streptozotocin induced type 2 diabetes in rats impacts osteogenesis and wnt signaling in bone marrow stromal cells. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. The males of various strains are differentially susceptible to developing diabetes. Injection of streptozotocin caused a threefold elevation of blood glucose by comparison with the control mice at four, six and eight weeks of diabetes. Streptozotocin induced diabetes provides a relevant example of. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural changes resulting in necrotic beta cell death. Experimentally induced diabetes through the administration of. In our experiments, we followed the rats with streptozotocin induced diabetes for one year and a recovery was observed in a significant portion of animals. Streptozotocininduced diabetic models in mice and rats.
1180 1481 944 1280 1189 1542 927 78 690 452 1542 1237 909 621 62 1302 1073 1544 876 1615 715 1447 767 1047 596 1423 83 1019 52 1195 127 1601 210 497 435 971 1344 560 194 154 631 1331 771